Human insulin can be produced by inserting the human insulin gene into the plasmid, putting the plasmid into bacteria, and putting the “recombinant” bacteria in fermentation tanks. There, the bacteria begin producing human insulin.
The recombinant human insulin can be produced by following steps:
1. The human insulin is extracted from pancreatic cells of humans and two genes are extracted that are responsible for insulin chain A and chain B production.
2. In the second step we extract a plasmid DNA from a bacteria and form a plasmid vector.
3. Then insert the human insulin chain A and B producing genes into this plasmid vector. This results in the formation of recombinants DNA.
4. The next step is introduction of recombinant DNA again into the bacterial cell. This results in the formation of the recombinant bacterium.
5. Then put the recombinant bacteria into the large fermentation tank where it multiplies. Each bacteria will produce human insulin chain A and chain B.
6. These A and B insulin chains are then extracted, purified and combined by creating disulfide bonds to form human insulin. And by this way, recombinant human insulin is produced.
