Question

Organelles can be separated from the homogenate cell by

• A. Autoradiography
• B. Differential centrifugation
• C. Chromatography
• D. X-ray diffraction

Answer 1

Answer:

B. Differential centrifugation

• Differential centrifugation is a well-known technique for separating organelles from a homogenate of disrupted cells.

• The principle involves the use of centrifugal force to take advantage of the different sedimentation rates of the organelles present.

• It is achieved by successive rounds of centrifugation at increasing speeds; the highest speed isolates the smallest and lightest organelle, while lower speeds separate the larger and denser elements, such as nuclei and cellular debris.

• Thus, this method allows various organelles such as mitochondria, lysosomes, peroxisomes, and microsomes to be segregated according to their respective size and density.