Question

The appropriate technique used for the rapid detection of specific sequences in an unpurified nucleic acid is ___________________.

• A. Southern blotting
• B. Northern blotting
• C. Western blotting
• D. Dot blotting

Answer 1

Answer:

D. Dot blotting

• Dot blotting is a simple and rapid method for detecting specific DNA or RNA sequences.

• It involves applying a small sample of nucleic acid to a membrane, which is then hybridized with a labeled probe that binds specifically to the target sequence.

• The resulting signal indicates the presence of the target sequence.