The markers revealing variations at DNA level are referred to as molecular markers. Which among the following molecular markers make the use of non- PCR-based approach?
ARFLP
BRAPD
CAFLP
DSSRs
Answer:
A. RFLP
Read Explanation:
RFLP (restriction fragment length polymorphism) method is a technique in which the DNA of various individuals is fragmented by restriction endonucleases and is separated by gel electrophoresis.
Restriction Fragment Length Polymorphism or RFLP is a non-PCR based approach.
The RFLP analysis differentiates or compares individuals based on polymorphisms in their genome.
These polymorphisms are detected by restriction digestion and the subsequent comparison of the lengths of the restriction fragments.
