Question

The markers revealing variations at DNA level are referred to as molecular markers. Which among the following molecular markers make the use of non- PCR-based approach?

• A. RFLP
• B. RAPD
• C. AFLP
• D. SSRs

Answer 1

Answer:

A. RFLP

• RFLP (restriction fragment length polymorphism) method is a technique in which the DNA of various individuals is fragmented by restriction endonucleases and is separated by gel electrophoresis.

• Restriction Fragment Length Polymorphism or RFLP is a non-PCR based approach.

• The RFLP analysis differentiates or compares individuals based on polymorphisms in their genome.

• These polymorphisms are detected by restriction digestion and the subsequent comparison of the lengths of the restriction fragments.