Question

The method used to identify the gene in Human Genome Project is:

• A. Open Reading Frame (ORF)
• B. Expressed Sequence Tag (EST)
• C. Linkage Disequilibrium Tag (LDT)
• D. Multiple Nucleotide Tag (MNT)

Answer 1

Answer:

B. Expressed Sequence Tag (EST)

HGP involved two major approaches:

• Expressed Sequence Tags (ESTs) – This approach focused on identifying all genes expressed as RNA.

• Sequence Annotation – This blind approach involved sequencing the whole genome (coding and non-coding) and later assigning functions to the different regions.