Question

The temperature cycles in a polymerase chain reaction are in the order _________________

• A. 95°, 60°, 72°
• B. 60°, 72°, 95°
• C. 72°, 60°, 95°
• D. 95°, 72°, 60°

Answer 1

Answer:

A. 95°, 60°, 72°

Three different temperatures are reached during the polymerase chain reaction to facilitate the denaturation and hybridization of the DNA strands. First the temperature is elevated to 95°C, then lowered to 60°C and then again increased to 72°C.