Question

ഡിഎൻഎ സീക്വൻസിങ് രീതികളെക്കുറിച്ച് ഇനിപ്പറയുന്നവയിൽ ഏതാണ് അസത്യം?

• A. Purified fragments of DNA cut from plasmid/phage clones or amplified by polymerase chain reaction (PCR)
• B. Clones of DNA fragments are denatured to single strands, and one of the strands is hybridized to an oligonucleotide primer
• C. Taq polymerase is quite heat sensitive
• D. New strands of DNA are synthesized from the end of the primer

Answer 1

Answer:

C. Taq polymerase is quite heat sensitive

In an automated procedure, new strands of DNA are synthesized from the end of the primer by heat-resistant Taq polymerase from a pool of deoxyribonucleotide triphosphates (dNTPs) that includes a small amount of one of four chain-terminating nucleotides (ddNTPs).