Question

_________________ enzyme is used for removing nucleotides from the 3' ends of dsDNA.

• A. S1 Nuclease
• B. λ - exonuclease
• C. Exonuclease III
• D. Bal 31 nuclease

Answer 1

Answer:

C. Exonuclease III

Exonuclease III is an enzyme that removes mononucleotides from the 3'-hydroxyl termini of double-stranded DNA (dsDNA) or DNA-RNA hybrids. It has several applications, including: Controlling deletion rate: Varying the incubation temperature can control the deletion rate. Generating nested deletions: Exonuclease III can generate nested sets of deletions in double-stranded, linear DNA. Preparing single-stranded DNA: Exonuclease III can be used to prepare single-stranded DNA.