Question

Various steps in downstream processing are given below. Arrange the season sequential order: (i) Extraction (ii) Cell destruction (iii) Drying (iv) Isolation (v) Purification (vi) Separation

• A. (i),(ii),(vi),(v),(iii),(iv)
• B. (iii),(vi),(i),(ii),(v),(iv)
• C. (iv),(i),(iii),(ii),(v),(vi)
• D. (vi),(ii),(i),(iv),(v),(iii)

Answer 1

Answer:

D. (vi),(ii),(i),(iv),(v),(iii)

Downstream processing steps include: Centrifugation A common step for clarifying harvested material on a small scale. Filtration A key step for capturing impurities and purifying samples. Precipitation A common technique used in more than 80% of recovery and purification methods. Purification A key step for improving product quality and purity. Ion exchange chromatography An important step for purifying target proteins from cell extracts. Adsorption A polishing process used in water treatment. Drying A stage of granulation that removes liquid from a finished product by applying heat.