Question

Which enzyme is active at 72° in the polymerase chain reaction?

• A. somerase
• B. exonuclease
• C. polymerase
• D. endonuclease

Answer 1

Answer:

C. polymerase

Taq polymerase, derived from the thermophilic bacteria Thermus aquaticus is active at 72°. The enzyme is responsible for synthesizing the complementary DNA strand by adding nucleotides to the 3’ end of the primers.